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Abbreviations CSE: Cigarette smoke extract TNF-: Tumor necrosis factor- EMT: Epithelial-mesenchymal transformation COPD: Chronic obstructive pulmonary disease CS: Cigarette smoke NAC: N -Acetylcysteine VWF: Von Willebrand factor MAPK: Mitogen-activated protein kinase FEV1: Forced expiratory volume in 1 FVC: Forced vital capacity PMSF: Phenylmethylsulphonyl fluoride ECL: Enhanced chemiluminescence SABC: Streptavidin biotin peroxidase complex ACK: Ammonium chloride-potassium FITC: Fluorescein isothiocyanate NC: Negative control DMEM: Dulbecco's modified Eagle's medium oe: Overexpression sh: Short hairpin RNA SD: SpragueDawley BCA: Bicinchoninic acid PEF: Peak expiratory flow HE: Hematoxylineosin RT-qPCR: Reverse transcription quantitative polymerase chain reaction GAPDH: Glyceraldehyde-3-phosphate dehydrogenase RIPA: Radio-immunoprecipitation assay SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis PVDF: Polyvinylidene fluoride ANOVA: Analysis of variance References Armstrong J, Harbron C, Lea S, Booth G, Cadden P, Wreggett KA, et al

If possible, avoid depressants including alcohol, benzodiazepines or opioids

It may take between 4 to 10 weeks of regular use for glutathione to show visible results (6)