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Taken together, these findings suggest that Nrf2 plays a vital role in mediating the chondroprotective effects of metformin by means of anti-oxidation, and that loss of Nrf2 substantially attenuates, but does not completely abolish, the therapeutic efficacy of metformin in the MIA-induced OA model

Given the pivotal role of biofilms in bacterial virulence and the establishment of persistent infections, these findings indicate a potential alteration in the pathogenic profile of the mutant strain, which may affect its ability to form protective communities against environmental stresses and antimicrobial agents

Reverse transcription-PCR confirmed the presence of a 109 bp intron and a transcript with two exons of 38 bp and 580 bp (Supplementary Figures S1B,C)

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