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These phosphorylated tyrosines serve as docking sites for adaptor proteins including insulin receptor substrate-1 (IRS-1), insulin receptor substrate-2 (IRS-2), and Shc, which are recruited to the activated receptor and themselves become phosphorylated, initiating multiple downstream signaling cascades

JAMA 315:24242434

The analysis steps were summarized as follows: (1) the extraction of total DNA from microbiota, (2) polymerase chain reaction (PCR) amplification, (3) purification and recovery, (4) fluorescence quantification, (5) sequencing library preparation, and (6) sequencing on the machine