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Phosphofructokinase-1 is the step exhibiting the highest flux-control coefficient in the pathway of glycolysis, thus representing the rate-limiting step (though this author advises against using the terminology rate-limiting step, as all steps in glycolysis and in any other pathway are rate-limiting, albeit to different extents)

[22] The method of the present invention is characterized by preparation of high-quality L-carnitine at a low production cost, through the development of a production process of alkyl (R)-4-chloro-3-hydroxybutyrate which is used as an intermediate

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